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Core genome MLST (cgMLST)-based genetic relatedness between 29 MCR1-EC isolates from South Korea. Data comprise 12 intestinal pathogenic MCR1-EC strains isolated in this study and 17 MCR1-EC strains isolated from South Korea, published in the National Center for Biotechnology Information (NCBI) GenBank database. The phylogenetic tree based on cgMLST was constructed using the neighbor-joining algorithm with default parameters implemented in the Ridom <t>SeqSphere+</t> program (v8.2.0). The color of shades corresponds to each clone type. The contents of shades include, from left to right, the assembly accession number, host, isolation date, clone type, and data source (this study or NCBI database) for each strain. Four clusters consist of MCR1-EC strains with a relatedness distance value less than 0.01. MCR1-EC, Escherichia coli carrying the mobilized colistin resistance gene mcr-1.1 ; MLST, multi-locus sequence typing.
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ <t>SeqSphere+</t> (v. 8.2.0) .
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Core genome MLST (cgMLST)-based genetic relatedness between 29 MCR1-EC isolates from South Korea. Data comprise 12 intestinal pathogenic MCR1-EC strains isolated in this study and 17 MCR1-EC strains isolated from South Korea, published in the National Center for Biotechnology Information (NCBI) GenBank database. The phylogenetic tree based on cgMLST was constructed using the neighbor-joining algorithm with default parameters implemented in the Ridom SeqSphere+ program (v8.2.0). The color of shades corresponds to each clone type. The contents of shades include, from left to right, the assembly accession number, host, isolation date, clone type, and data source (this study or NCBI database) for each strain. Four clusters consist of MCR1-EC strains with a relatedness distance value less than 0.01. MCR1-EC, Escherichia coli carrying the mobilized colistin resistance gene mcr-1.1 ; MLST, multi-locus sequence typing.

Journal: Frontiers in Microbiology

Article Title: Prevalence, Characteristics, and Clonal Distribution of Escherichia coli Carrying Mobilized Colistin Resistance Gene mcr-1.1 in Swine Farms and Their Differences According to Swine Production Stages

doi: 10.3389/fmicb.2022.873856

Figure Lengend Snippet: Core genome MLST (cgMLST)-based genetic relatedness between 29 MCR1-EC isolates from South Korea. Data comprise 12 intestinal pathogenic MCR1-EC strains isolated in this study and 17 MCR1-EC strains isolated from South Korea, published in the National Center for Biotechnology Information (NCBI) GenBank database. The phylogenetic tree based on cgMLST was constructed using the neighbor-joining algorithm with default parameters implemented in the Ridom SeqSphere+ program (v8.2.0). The color of shades corresponds to each clone type. The contents of shades include, from left to right, the assembly accession number, host, isolation date, clone type, and data source (this study or NCBI database) for each strain. Four clusters consist of MCR1-EC strains with a relatedness distance value less than 0.01. MCR1-EC, Escherichia coli carrying the mobilized colistin resistance gene mcr-1.1 ; MLST, multi-locus sequence typing.

Article Snippet: The cgMLST was performed using the Ridom SeqSphere+ program (v8.2.0; ).

Techniques: Isolation, Construct, Sequencing

Core genome multi-locus sequence typing-based genetic relatedness of 82 ST10-A MCR1-EC strains isolated from humans, pigs, and chickens worldwide. Strains were derived from the NCBI GenBank database. The phylogenetic tree based on cgMLST was constructed using the neighbor-joining algorithm with default parameters implemented in the Ridom SeqSphere+ program (v8.2.0). The shaded color corresponds to the country where the strain was isolated. The contents of shades include, from left to right, the assembly accession number, host, isolation date, isolation country, and data source (this study or NCBI database) for each strain. Five clusters consist of MCR1-EC isolates with a relatedness distance value of less than 0.01. MCR1-EC, Escherichia coli carrying the mobilized colistin resistance gene mcr-1.1 ; cgMLST, core genome multi-locus sequence typing.

Journal: Frontiers in Microbiology

Article Title: Prevalence, Characteristics, and Clonal Distribution of Escherichia coli Carrying Mobilized Colistin Resistance Gene mcr-1.1 in Swine Farms and Their Differences According to Swine Production Stages

doi: 10.3389/fmicb.2022.873856

Figure Lengend Snippet: Core genome multi-locus sequence typing-based genetic relatedness of 82 ST10-A MCR1-EC strains isolated from humans, pigs, and chickens worldwide. Strains were derived from the NCBI GenBank database. The phylogenetic tree based on cgMLST was constructed using the neighbor-joining algorithm with default parameters implemented in the Ridom SeqSphere+ program (v8.2.0). The shaded color corresponds to the country where the strain was isolated. The contents of shades include, from left to right, the assembly accession number, host, isolation date, isolation country, and data source (this study or NCBI database) for each strain. Five clusters consist of MCR1-EC isolates with a relatedness distance value of less than 0.01. MCR1-EC, Escherichia coli carrying the mobilized colistin resistance gene mcr-1.1 ; cgMLST, core genome multi-locus sequence typing.

Article Snippet: The cgMLST was performed using the Ridom SeqSphere+ program (v8.2.0; ).

Techniques: Sequencing, Isolation, Derivative Assay, Construct

( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ SeqSphere+ (v. 8.2.0) .

Journal: Antibiotics

Article Title: Clostridioides difficile in South American Camelids in Germany: First Insights into Molecular and Genetic Characteristics and Antimicrobial Resistance

doi: 10.3390/antibiotics12010086

Figure Lengend Snippet: ( a ) Results of core genome multi locus sequence typing (cgMLST) of C. difficile isolates. Numbers next to the branches indicate numbers of allelic differences in the core genome. In brackets, the number of SNPs within the core genome is indicated. cgMLST classified the eight isolates into five genetic groups. Three isolates were singletons, while the other five isolates were grouped into two clusters (marked in grey) with two and three isolates, respectively. Each node represents a cgMLST sequence type (cgST) which was coloured according to their ST (classical MLST) classification. ( b ) Geographic origin (according to postal code) of the corresponding samples to the isolates. 19S0160 and 19S0161 (green; RT 015) originated from the same husbandry. RT 002/2 isolates (19S0260, 19S0162, 19S0264; red) originated from husbandries in Saxony-Anhalt which were close to each other, as did 19S0266 (purple; RT 029). 19S0136 (blue; RT 078) originated from a husbandry in Saxony. Figures were created with Ridom™ SeqSphere+ (v. 8.2.0) .

Article Snippet: All tools were used with default settings. cgMLST analysis was done with Ridom SeqSphere+ ™(v. 8.2.0) [ ].

Techniques: Sequencing